Anti-PHF1 antibody (ab80042)
Key features and details
- Rabbit polyclonal to PHF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHF1 antibody
See all PHF1 primary antibodies -
Description
Rabbit polyclonal to PHF1 -
Host species
Rabbit -
Specificity
This antibody is specific to PHD finger protein 1 -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human Heart Tissue Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. -
Tissue specificity
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney. -
Sequence similarities
Contains 2 PHD-type zinc fingers. -
Cellular localization
Nucleus. Localizes specifically to the promoters of numerous target genes. - Information by UniProt
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Database links
- Entrez Gene: 508957 Cow
- Entrez Gene: 5252 Human
- Entrez Gene: 21652 Mouse
- Entrez Gene: 294287 Rat
- Omim: 602881 Human
- SwissProt: O43189 Human
- SwissProt: Q9Z1B8 Mouse
- Unigene: 166204 Human
see all -
Alternative names
- hPCl1 antibody
- MTF2 L2 antibody
- MTF2L 2 antibody
see all
Images
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Western blot - Anti-PHF1 antibody (ab80042)Anti-PHF1 antibody (ab80042) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 50 kDa (possible isoform)
Exposure time: 3 minutes
This antibody is predicted to cross react with both isoform 1 (50-kDa) and isoform 2 (62-kDa) of PHF1. We believe that the band observed at 50-kDa corresponds to isoform 1. -
Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody (ab80042)ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab80042 has been referenced in 5 publications.
- Dong C et al. Structural basis for histone variant H3tK27me3 recognition by PHF1 and PHF19. Elife 9:N/A (2020). PubMed: 32869745
- Wang L et al. Deep cervical lymph node ligation aggravates AD-like pathology of APP/PS1 mice. Brain Pathol 29:176-192 (2019). PubMed: 30192999
- Zhang R et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31364823
- Qu Y et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). PubMed: 30382790
- Liu R et al. PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis. Nucleic Acids Res 46:6608-6626 (2018). PubMed: 29846670
Images
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Western blot - Anti-PHF1 antibody (ab80042)Anti-PHF1 antibody (ab80042) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 50 kDa (possible isoform)
Exposure time: 3 minutes
This antibody is predicted to cross react with both isoform 1 (50-kDa) and isoform 2 (62-kDa) of PHF1. We believe that the band observed at 50-kDa corresponds to isoform 1. -
Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody (ab80042)ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.
