Recombinant human EZH2 + EED + SUZ12 protein (ab196409)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 82% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human EZH2 + EED + SUZ12 protein -
Biological activity
Specific Activity: 0.08 pmole/min/µg.
Assay conditions; 50 µl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 µM S-adenosylmethionine, and 250-2000 ng ab196409 is added to the wells coated with histone H3 substrate on a Neutravidin black plate (ThermoFisher). Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Add HRP chemiluminescent substrates and read luminescence.
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Purity
>= 82 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
SEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTE RPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQF NWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCA WTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPR DPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKI MSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFST RDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVT ILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKC TTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR -
Predicted molecular weight
51 kDa including tags -
Amino acids
2 to 441 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
EED; NM_003797.
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Amino Acid Sequence 2
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Species
Human -
Sequence
APQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGS YSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLR TRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESH SLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSC PIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLL FRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTV FDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQ GPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIA VKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCP WCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAG NPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEV EQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIE EFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKN LCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEI TEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL -
Predicted molecular weight
87 kDa including tags -
Amino acids
2 to 739 -
Tags
His tag N-Terminus -
Additional sequence information
SUZ12; NM_015355.
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Amino Acid Sequence 3
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Species
Human -
Sequence
GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILE RTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTL NAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIK NYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQK DLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQ LPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKCN YSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTP PKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKE EEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGT YYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKH RLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKF CQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDS KNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEII SQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCY AKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP -
Predicted molecular weight
86 kDa including tags -
Amino acids
2 to 751 -
Tags
His tag N-Terminus -
Additional sequence information
EZH2; NM_004456.
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Preparation and Storage
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-SUZ12 antibody (ab12073)
- Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade (ab175187)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-KMT6 / EZH2 antibody (ab186006)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Images
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Functional Studies - Recombinant human EZH2 + EED + SUZ12 protein (ab196409)
Activity assay of ab196409.
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SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 protein (ab196409)4-20% SDS-PAGE analysis of 3.9 µg ab196409 with Coomassie staining.