Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)
Key features and details
- Rabbit polyclonal to Phospholipase C beta 3/PLCB3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C beta 3/PLCB3 antibody
See all Phospholipase C beta 3/PLCB3 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C beta 3/PLCB3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Phospholipase C beta 3/PLCB3 aa 850-1000.
Database link: Q01970-1 -
Positive control
- IHC-P: Human small intestine and duodenum tissue. ICC/IF: MCF7 cells. WB: RT4 and U-251 MG cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)
PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Phospholipase C beta 3/PLCB3 (green) using ab251862 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)Paraffin-embedded human small intestine tissue stained for Phospholipase C beta 3/PLCB3 using ab251862 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)Paraffin-embedded human duodenum tissue stained for Phospholipase C beta 3/PLCB3 using ab251862 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)Paraffin-embedded human testis tissue stained for Phospholipase C beta 3/PLCB3 using ab251862 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)Paraffin-embedded human lymph node tissue stained for Phospholipase C beta 3/PLCB3 using ab251862 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)Paraffin-embedded human cerebral cortex tissue stained for Phospholipase C beta 3/PLCB3 using ab251862 at 1/500 dilution in immunohistochemical analysis. Low expression as expected.
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Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862)All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody (ab251862) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 139 kDa
