Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COL18A1 antibody [EPR23481-21] (ab275746)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23481-21] to COL18A1
  • Suitable for: IP, WB, ICC, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-COL18A1 antibody [EPR23481-21]
    See all COL18A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23481-21] to COL18A1

  • Host species

    Rabbit

  • Specificity

    This antibody does not recognise Endostatin.

    This antibody (ab275746 [EPR23481-21] is a distinct clone from ab275390 [EPR23481-240].

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and Huh7 whole cell lysates. IHC-P: Human kidney and bladder cancer tissue. ICC: HepG2 cells. Flow cyt: HepG2 cells. IP: Huh7 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23481-21

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL18A1 antibody [EPR23481-21] (ab275746)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COL18A1 with ab275746 at 1/4000 (0.134 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney (PMID: 12588956). The section was incubated with ab275746 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    Flow Cytometry - Anti-COL18A1 antibody [EPR23481-21] (ab275746)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U-937 (Human histiocytic lymphoma monocyte, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling COL18A1 with ab275746 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: U937 cell (PMID: 15451191).

  • Immunocytochemistry - Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    Immunocytochemistry - Anti-COL18A1 antibody [EPR23481-21] (ab275746)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling COL18A1 with ab275746 at 1/50 (8.26 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000  dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cells. Negative control: U937 cell (PMID: 15451191).  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    Western blot - Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    All lanes : Anti-COL18A1 antibody [EPR23481-21] (ab275746) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : Huh7 (human hepatocellar carcinoma epithelial cell), whole cell lysate at 40 µg
    Lane 3 : U937 (human histiocytic lymphoma monocyte), whole cell lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 178 kDa
    Observed band size: 178 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 11581192, 15451191).

    Negative control: U937 (PMID:15451191).

    The lanes 1 of  blot were developed using a higher sensitivity ECL substrate.

    Exposure time: Lane 1: 3 minutes Lane 2-3: 70 seconds.

     

  • Immunoprecipitation - Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    Immunoprecipitation - Anti-COL18A1 antibody [EPR23481-21] (ab275746)

    COL18A1 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate with ab275746 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275746 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab275746 IP in Huh7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of 275746 in Huh7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL18A1 antibody [EPR23481-21] (ab275746)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling COL18A1 with ab275746 at 1/4000 (0.134 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of human bladder cancer (PMID:21815140). The section was incubated with ab275746 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-COL18A1 antibody [EPR23481-21] (ab275746)
    Anti-COL18A1 antibody [EPR23481-21] (ab275746)

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