Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SVH antibody [EPR13763] - C-terminal (ab188573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13763] to SVH - C-terminal
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SVH antibody [EPR13763] - C-terminal
    See all SVH primary antibodies

  • Description

    Rabbit monoclonal [EPR13763] to SVH - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SKBR-3, A431, HepG2, U87-MG, C6, Raw264.7, PC-12, NIH3T3 lysates; A431 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)
    Western blot - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)
    All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilution

    Lane 1 : SKBR-3 lysate
    Lane 2 : A431 lysate
    Lane 3 : HepG2 lysate
    Lane 4 : U87-MG lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38, 34, 31, 27, 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)
    Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431cells labeling SVH with ab188573 at 1/50 followed by Goat anti rabbit IgG (Alexa Fluor®488) and DAPI staining.

  • Western blot - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)
    Western blot - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)
    All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilution

    Lane 1 : C6 lysate
    Lane 2 : RAW264.7 lysate
    Lane 3 : PC-12 lysate
    Lane 4 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38, 34, 31, 27, 31 kDa

  • Immunoprecipitation - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)
    Immunoprecipitation - Anti-SVH antibody [EPR13763] - C-terminal (ab188573)

    Western blot analysis of SVH in immunoprecipitation pellets from U87-MG lysate. ab188573 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.



    All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilution

    Lane 1 : U87-MG
    Lane 2 : PBS instead of U87-MG lysates

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Additional bands at: 27, 34 kDa. We are unsure as to the identity of these extra bands.

  • Anti-SVH antibody [EPR13763] - C-terminal (ab188573)
    Anti-SVH antibody [EPR13763] - C-terminal (ab188573)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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