Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18466] to KDM4A / JHDM3A / JMJD2A - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free
    See all KDM4A / JHDM3A / JMJD2A primary antibodies

  • Description

    Rabbit monoclonal [EPR18466] to KDM4A / JHDM3A / JMJD2A - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC application is recommended for human and rat.

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human KDM4A/ JHDM3A/ JMJD2A aa 550-650. The exact sequence is proprietary.
    Database link: O75164

  • General notes

    Ab251084 is the carrier-free version of ab191433. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251084 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Western blot - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)

    This data was developed using ab191433, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: DM4A / JHDM3A / JMJD2A knockout HAP1 cell lysate (20 µg) 

    Lane 3: 2OS cell lysate (20 µg)

    Lane 4: C12 cell lysate (20 µg)

    Lanes 1 - 4: erged signal (red and green). Green - ab191433 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab191433 was shown to specifically react with KDM4A / JHDM3A / JMJD2A when KDM4A / JHDM3A / JMJD2A knockout samples were used. Wild-type and KDM4A / JHDM3A / JMJD2A knockout samples were subjected to SDS-PAGE. ab191433 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)

    This data was developed using ab191433, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling KDM4A / JHDM3A / JMJD2A with ab191433 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining in epithelial cells of human colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Immunoprecipitation - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)

    This data was developed using ab191433, the same antibody clone in a different buffer formulation.

    KDM4A / JHDM3A / JMJD2A was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab191433 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab191433 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HeLa whole cell lysate 10µg (Input). Lane 2: ab191433 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191433 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.
  • Western blot - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Western blot - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Lanes 1-3 : Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] (ab191433) at 1/5000 dilution
    Lane 4 : Anti-MTHFD2 antibody [7] (ab191443) at 1/5000 dilution

    Lane 1 : Human fetal kidney at 10 µg
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
    Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg
    Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole call lysate at 20 µg

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 120 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab191433, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1, 3-4: 1 minute; Lane 2: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)

    This data was developed using ab191433, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling KDM4A / JHDM3A / JMJD2A with ab191433 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining in epithelial cells of rat colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Western blot - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    All lanes : Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] (ab191433) at 1/5000 dilution

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 120 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab191433, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)
    Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] - BSA and Azide free (ab251084)

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