Anti-FOXO1A (phospho S322 + S325) antibody (ab60945)
Key features and details
- Rabbit polyclonal to FOXO1A (phospho S322 + S325)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-FOXO1A (phospho S322 + S325) antibody
See all FOXO1A primary antibodies -
Description
Rabbit polyclonal to FOXO1A (phospho S322 + S325) -
Host species
Rabbit -
Specificity
ab60945 detects endogenous levels of FOXO1A only when phosphorylated at serine 322 and serine 325. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 322 and serine 325 (N-A-SP-T-I-SP-G-R).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab60945 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FOXO1A (phospho S322 + S325) antibody (ab60945)All lanes : Anti-FOXO1A (phospho S322 + S325) antibody (ab60945) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts treated with Wortmannin (40nM, 24hours)
Lane 2 : NIH 3T3 cell extracts treated with Wortmannin (40nM, 24hours) with immunising peptide
Predicted band size: 70 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
