Anti-ARID1A antibody [CL3595] (ab242377)
![Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-386734-anti-arid1A-antibody-cl3595-immunofluorescence-a431-cells-human.jpg "Anti-ARID1A antibody [CL3595] (ab242377)")
Key features and details
- Mouse monoclonal [CL3595] to ARID1A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ARID1A antibody [CL3595]
See all ARID1A primary antibodies -
Description
Mouse monoclonal [CL3595] to ARID1A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human ARID1A aa 1250-1400.
Database link: O14497 -
Epitope
Binds to an epitope located within the peptide sequence VAMGPRQHYPYGGPY as determined by overlapping synthetic peptides. -
Positive control
- WB: ARID1A over-expression (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK-293T). IHC-P: Human breast cancer, prostate cancer, liver cancer, fallopian tube, colon and kidney tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
purified from TCS. -
Clonality
Monoclonal -
Clone number
CL3595 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [CL3595] (ab242377)
PFA-fixed, Triton X-100 permeabilized A431 cells stained for ARID1A (Green) using AB242377 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
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![Western blot - Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-331616-anti-arid1a-antibody-cl3595-western-blot.jpg)
Western blot - Anti-ARID1A antibody [CL3595] (ab242377)All lanes :
Lane 1 : Empty vector-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : ARID1A over-expression (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK-293T), cell lysate
Predicted band size: 242 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-331617-anti-arid1a-antibody-cl3595-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)Paraffin-embedded human breast cancer tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-331618-anti-arid1a-antibody-cl3595-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)Paraffin-embedded human prostate cancer tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-331619-anti-arid1a-antibody-cl3595-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)Paraffin-embedded human liver cancer tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-331620-anti-arid1a-antibody-cl3595-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)Paraffin-embedded human fallopian tube tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-331621-anti-arid1a-antibody-cl3595-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)Paraffin-embedded human colon tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)](https://www.abcam.com/ps/products/242/ab242377/Images/ab242377-331622-anti-arid1a-antibody-cl3595-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (ab242377)Paraffin-embedded human kidney tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.
