Recombinant human KDM5C / Jarid1C / SMCX protein (ab196392)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 85% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human KDM5C / Jarid1C / SMCX protein
See all KDM5C / Jarid1C / SMCX proteins and peptides -
Biological activity
Specific activity of 0.078 pmol/min/µg.
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Purity
>= 85 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
EPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPAD WQPPFAVEV DNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSS LKIPNVERRILDLYSLSKIVV EEGGYEAICKDRRWARVAQRLNYPPGK NIGSLLRSHYERIVYPYEMYQSGANLVQCNTRP FDNEEKDKEYKPHSI PLRQSVQPSKFNSYGRRAKRLQPDPEPTEEDIEKNPELKKLQIYG AGP KMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVKVESTSPKTFLE SKEELSH SPEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLL CDGCDDNYHIFCLLPPLPE IPKGVWRCPKCVMAECKRPPEAFGFEQAT REYTLQSFGEMADSFKADYFNMPVHMVPTEL VEKEFWRLVNSIEEDVT VEYGADIHSKEFGSGFPVSDSKRHLTPEEEEYATSGWNLNVMP VLEQS VLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWY GVPSL AAEHLEEVMKKLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPV VRTNQCAGEFVITFPRA YHSGFNQGYNFAEAVNFCTADWLPAGRQCIE HYRRLRRYCVFSHEELICKMAACPEKLDL NLAAAVHKEMFIMVQEERR LRKALLEKGITEAEREAFELLPDDERQCIKCKTTCFLSALA CYDCPDG LVCLSHINDLCKCSSSRQYLRYRYTLDELPAMLHKLKVRAESFDTWANKV RVA LEVEDGRKRSLEELRALESEARERRFPNSELLQQLKNCLSEAEAC VSRALGLVSGQEAGP HRVAGLQMTLTELRAFLDQMNNLPCAMHQIGDV KGVLEQVEAYQAEAREALASLPSSPGL LQSLLERGRQLGVEVPEAQQL QRQVEQARWLDEVKRTLAPSARRGTLAVMRGLLVAGASV APSPAVDKA QAELQELLTIAERWEEKAHLCLEARQKHPPATLEAIIREAENIPVHLPNI Q ALKEALAKARAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLPVGLEE LRQLELQVLTAHS WREKASKTFLKKNSCYTLLEVLCPCADAGSDSTKR SRWMEKELGLYKSDTELLGLSAQDL RDPGSVIVAFKEGEQKEKEGILQ LRRTNSAKPSPLASSSTASSTTSICVCGQVLAGAGAL QCDLCQDWFHG RCVSVPRLLSSPRPNPTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILA LLVALQRLPVRLPEGEALQCLTERAISWQGRARQALASEDVTALLGRLA ELRQRLQAEPR PEEPPNYPAAPASDPLREGSGKDMPKVQGLLENGDSV TSPEKVAPEEGSGKRDLELLSSL LPQLTGPVLELPEATRAPLEELMME GDLLEVTLDENHSIWQLLQAGQPPDLERIRTLLEL EKAERHGSRARGR ALERRRRRKVDRGGEGDDPAREELEPKRVRSSGPEAEEVQEEEELEE E TGGEGPPAPIPTTGSPSTQENQNGLEPAEGTTSGPSAPFSTLTPRLHLPC PQQPPQQQL -
Predicted molecular weight
181 kDa including tags -
Amino acids
2 to 1560 -
Tags
His tag N-Terminus , DDDDK tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 64% Sodium chloride, 0.02% Potassium chloride, 0.462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
Also contains 80 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human KDM5C / Jarid1C / SMCX protein (ab196392)
10 µL reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196392 (50 – 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.
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SDS-PAGE - Recombinant human KDM5C / Jarid1C / SMCX protein (ab196392)10% SDS PAGE with Coomassie staining: 6 µg ab196392.
