Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RRAGA + RRAGB antibody (ab91062)

Key features and details

  • Rabbit polyclonal to RRAGA + RRAGB
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RRAGA + RRAGB antibody

  • Description

    Rabbit polyclonal to RRAGA + RRAGB

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Synthetic peptide corresponding to Human RRAGA + RRAGB aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91427)

  • Positive control

    • This antibody gave a positive signal in the following while cell lysates: HeLa; Jurkat; HEK293; Raji; SHSY-5Y.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab91062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).

Target

  • Relevance

    Involved in the RCC1/Ran-GTPase pathway. RRAGA may play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. biquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.

  • Cellular localization

    Cytoplasmic and Nuclear: RRAGA may shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state and colocalizes in vivo with adenovirus E3-14.7K. RRAGB is cytoplasmic.

  • Database links

    • Alternative names

      • Adenovirus E3 14.7 kDa-interacting protein 1 antibody
      • FIP1 antibody
      • Rag A antibody
      • RAg B antibody
      • RagA antibody
      • RAGB antibody
      • Ras related GTP binding protein A antibody
      • Ras-related GTP binding protein B antibody
      see all

    Images

    • Western blot - Anti-RRAGA + RRAGB antibody (ab91062)
      Western blot - Anti-RRAGA + RRAGB antibody (ab91062)
      All lanes : Anti-RRAGA + RRAGB antibody (ab91062) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 37 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-RRAGA + RRAGB antibody (ab91062)
      Immunocytochemistry/ Immunofluorescence - Anti-RRAGA + RRAGB antibody (ab91062)
      ICC/IF image of ab91062 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91062, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRAGA + RRAGB antibody (ab91062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRAGA + RRAGB antibody (ab91062)
      IHC image of RRAGA + RRAGB staining in human normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91062, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    References (0)

    Publishing research using ab91062? Please let us know so that we can cite the reference in this datasheet.

    ab91062 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-RRAGA + RRAGB antibody (ab91062)
      Western blot - Anti-RRAGA + RRAGB antibody (ab91062)
      All lanes : Anti-RRAGA + RRAGB antibody (ab91062) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 37 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-RRAGA + RRAGB antibody (ab91062)
      Immunocytochemistry/ Immunofluorescence - Anti-RRAGA + RRAGB antibody (ab91062)
      ICC/IF image of ab91062 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91062, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRAGA + RRAGB antibody (ab91062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRAGA + RRAGB antibody (ab91062)
      IHC image of RRAGA + RRAGB staining in human normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91062, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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