Anti-ASC-2/NRC antibody (ab18193)
Key features and details
- Rabbit polyclonal to ASC-2/NRC
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ASC-2/NRC antibody
See all ASC-2/NRC primary antibodies -
Description
Rabbit polyclonal to ASC-2/NRC -
Host species
Rabbit -
Specificity
This antibody was tested on U937, human prostate, human ovary and human testis extracts in western blot analysis and did not detect ASC-2/NRC in these extracts.
-
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
-
Positive control
- This antibody gives a positive signal in HeLa, HepG2 and MCF7 nuclear lysates.
-
General notes
This product was previously labelled as ASC-2
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).Target
-
Relevance
The protein encoded by this gene is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone dependent fashion. It is involved in the coactivation of different nuclear receptors, such as steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). It probably functions as a general coactivator, rather than just a nuclear receptor coactivator,and may also be involved in the coactivation of the NFkB pathway (referenced from swissprot). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 23054 Human
- Omim: 605299 Human
-
Alternative names
- Activating signal cointegrator 2 antibody
- AIB3 antibody
- Amplified in breast cancer 3 protein antibody
see all
Images
-
Western blot - Anti-ASC-2/NRC antibody (ab18193)All lanes : Anti-ASC-2/NRC antibody (ab18193) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 220 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 210 kDa, 65 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18193 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (4)
ab18193 has been referenced in 4 publications.
- Dai W et al. Melatonin prevents sepsis-induced renal injury via the PINK1/Parkin1 signaling pathway. Int J Mol Med 44:1197-1204 (2019). PubMed: 31432108
- Ying Y et al. NLRP3 Inflammasome Activation by MicroRNA-495 Promoter Methylation May Contribute to the Progression of Acute Lung Injury. Mol Ther Nucleic Acids 18:801-814 (2019). PubMed: 31734560
- Han SI et al. Estrogen receptor ligands ameliorate fatty liver through a nonclassical estrogen receptor/Liver X receptor pathway in mice. Hepatology 59:1791-802 (2014). PubMed: 24277692
- Massip L et al. Deciphering the chromatin landscape induced around DNA double strand breaks. Cell Cycle 9:2963-72 (2010). PubMed: 20714222
Images
-
Western blot - Anti-ASC-2/NRC antibody (ab18193)All lanes : Anti-ASC-2/NRC antibody (ab18193) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 220 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 210 kDa, 65 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18193 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
