Anti-Cdc25A (phospho S178) antibody (ab79252)
Key features and details
- Rabbit polyclonal to Cdc25A (phospho S178)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cdc25A (phospho S178) antibody
See all Cdc25A primary antibodies -
Description
Rabbit polyclonal to Cdc25A (phospho S178) -
Host species
Rabbit -
Specificity
Detects Cdc25A only when phosphorylated at serine 178 -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic phosphopeptide derived from Human Cdc25A around the phosphorylation site of serine 178 (Q-N-SP-A-P)
-
Positive control
- WB: extracts from UV-treated A549 cells; IHC-P: Human breast carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S178) antibody (ab79252)Left panel: immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using 1/50 ab79252. Right panel: same, after pre-incubation with the immunizing peptide.
-
Western blot - Anti-Cdc25A (phospho S178) antibody (ab79252)All lanes : Anti-Cdc25A (phospho S178) antibody (ab79252) at 1/500 dilution
Lane 1 : Extracts from A549 cell treated
with UV (15mins)
Lane 2 : Extracts from A549 cell treated
with UV (15mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 110 kDa, 117 kDa. We are unsure as to the identity of these extra bands.
