Anti-Cdc25A (phospho S76) antibody (ab75743)
Key features and details
- Rabbit polyclonal to Cdc25A (phospho S76)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc25A (phospho S76) antibody
See all Cdc25A primary antibodies -
Description
Rabbit polyclonal to Cdc25A (phospho S76) -
Host species
Rabbit -
Specificity
ab75743 detects endogenous levels of Cdc25A only when phosphorylated at serine 76. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human Cdc25A around the phosphorylation site of serine 76 (M-G-SP-S-E).
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Positive control
- Human breast carcinoma tissue; extracts from A2780 cells untreated or treated with UV.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75743 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S76) antibody (ab75743)ab75743, at a 1/50 dilution, staining Cdc25A in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
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Western blot - Anti-Cdc25A (phospho S76) antibody (ab75743)All lanes : Anti-Cdc25A (phospho S76) antibody (ab75743) at 1/500 dilution
Lane 1 : A2780 cell extracts, untreated
Lane 2 : A2780 cell extracts, treated with UV
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
