All lanes : Anti-ATG14L antibody (ab277796) at 3 µg/ml

Lane 1 : HeLa serum starved and treated with Rapamycin (100nM/4 hours) whole cell extract
Lane 2 : HeLa Serum starved whole cell extracts
Lane 3 : HeLa whole cell extract
Lane 4 : A549 serum starved treated with Rapamycin (100nM/4 hours) whole cell extract
Lane 5 : A549 serum starved whole cell extract
Lane 6 : A549 whole cell extract
Lane 7 : U-87-MG serum starved and treated with Rapamycin (100nM/4 hours) whole cell extract
Lane 8 : U-87-MG whole cell extract

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate, 0.4 µg/mL at 1/2500 dilution

Predicted band size: 55 kDa