Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8888] to Cdc25A (phospho S124)
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-Cdc25A (phospho S124) antibody [EPR8888]
See all Cdc25A primary antibodies
Description

Rabbit monoclonal [EPR8888] to Cdc25A (phospho S124)
Host species

Rabbit
Specificity

ab156574 only detects Cdc25A phosphorylated at Serine 124.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Serine 124 of Human Cdc25A (UniProt ID: P30304).
Positive control
- HeLa cell lysate; Human gastric carcinoma and Human testis tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8888
Isotype

IgG
Research areas

Western blot - Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574)

All lanes : Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574) at 1/1000 dilution

Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 59 kDa
Immunoprecipitation - Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574)

ab156574, at a 1/1000 dilution, staining Cdc25A (phospho S124) in HeLa lysate immunoprecipitated using ab156574 at 1/10 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574)

Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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