FOXO1 Transcription Factor Assay Kit (Colorimetric) (ab207204)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Nuclear Extracts
- Sensitivity: 5000 ng/well
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 5 x 96 tests 10X Antibody Binding Buffer 1 x 2.2ml 1 x 22ml 10X Wash Buffer 1 x 22ml 1 x 110ml 96-well FKHR assay plate 1 unit 5 units Anti-rabbit HRP-conjugated IgG (0.25 μg/μL) 1 x 11µl 1 x 55µl Binding Buffer 1 x 10ml 1 x 50ml Developing Solution 1 x 11ml 1 x 55ml Dithiothreitol (DTT) (1 M) 1 x 100µl 1 x 500µl FKHR (FOXO1) antibody 1 x 12µl 1 x 60µl Herring sperm DNA (1 μg/μL) 1 x 100µl 1 x 500µl Lysis Buffer 1 x 10ml 1 x 50ml Mutated oligonucleotide (10 pmol/µL) 1 x 100µl 1 x 500µl Plate sealer 1 unit 5 units Protease Inhibitor Cocktail 1 x 100µl 1 x 500µl Raji nuclear extract (2.5 µg/µL) 1 x 40µl 1 x 200µl Stop Solution 1 x 11ml 1 x 55ml Wild-type oligonucleotide (10 pmol/µL) 1 x 100µl 1 x 500µl -
Research areas
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Function
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsPhosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Alternative names
- FKH 1
- FKH1
- FKHR
see all -
Database links
- Entrez Gene: 2308 Human
- Entrez Gene: 56458 Mouse
- Entrez Gene: 84482 Rat
- Omim: 136533 Human
- SwissProt: Q12778 Human
- SwissProt: Q9R1E0 Mouse
- SwissProt: G3V7R4 Rat
- Unigene: 370666 Human
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Images
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Different amounts of nuclear extracts from untreated Raji (Black) and HeLa (Gray) cells are tested for FKHR activity. These results are provided for demonstration purposes only.
Different amounts of nuclear extracts from untreated HeLa (grey) and Raji (black) cells were tested for FOXO1 activity. These results are provided for demonstration purposes only.