Porcine Angiopoietin-1 ELISA Kit (ab273219)
Key features and details
- Sensitivity: 0.06 ng/ml
- Range: 0.061 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine Angiopoietin-1 ELISA Kit
See all Angiopoietin 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.06 ng/ml -
Range
0.061 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87.09 81% - 91% Plasma 73.02 67% - 80% Cell culture media 99.1 90% - 111% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine Angiopoietin-1 ELISA Kit (ab273219) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Angiopoietin-1 in serum (porcine Angiopoietin-1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for porcine Angiopoietin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-porcine Angiopoietin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine Angiopoietin-1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine Angiopoietin-1 Antibody 2 vials Porcine Angiopoietin-1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AGP 1
- AGP1
- AGPT
see all -
Database links
- Entrez Gene: 397009 Pig
- SwissProt: Q9BDY8 Pig
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
