Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)
![Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)](https://www.abcam.com/ps/products/250/ab250098/Images/ab250098-3-anti-smchd1-antibody-epr12340-29-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12340-29] to SMCHD1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free
See all SMCHD1 primary antibodies -
Description
Rabbit monoclonal [EPR12340-29] to SMCHD1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment within Human SMCHD1 aa 1800-2000. The exact sequence is proprietary.
Database link: A6NHR9 -
General notes
Ab250098 is the carrier-free version of ab179456. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250098 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12340-29 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)This data was developed using ab179456, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human uterus tissue, labeling SMCHD1 using ab179456 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)](https://www.abcam.com/ps/products/250/ab250098/Images/ab250098-2-anti-smchd1-antibody-epr12340-29-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)This data was developed using ab179456, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast tissue, labeling SMCHD1 using ab179456 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)](https://www.abcam.com/ps/products/250/ab250098/Images/ab250098-398178-anti-smchd1-antibody-epr12340-29-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)All lanes : Anti-SMCHD1 antibody [EPR12340-29] (ab179456) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : U-87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 226 kDaThis data was developed using ab179456, the same antibody clone in a different buffer formulation.
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![Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)](https://www.abcam.com/ps/products/250/ab250098/Images/ab250098-4-benefits-of-recombinant-antibodies.png)
Anti-SMCHD1 antibody [EPR12340-29] - BSA and Azide free (ab250098)