Human TMED10 (TMP21) knockout HEK-293T cell line (ab266227)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Purity
Immunogen affinity purified -
Research areas
Images
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Western blot - Human TMED10 knockout HEK293T cell line (ab266227)All lanes : Anti-TMP21 antibody [EPR9036(B)] (ab134948) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TMED10 knockout HEK293T cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab134948 observed at 19 kDa. Red - loading control ab8245 observed at 36 kDa.
ab134948 Anti-TMP21 antibody [EPR9036(B)] was shown to specifically react with TMP21 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266227 (knockout cell lysate ab258233) was used. Wild-type and TMP21 knockout samples were subjected to SDS-PAGE. ab134948 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human TMED10 knockout HEK293T cell line (ab266227)All lanes : Anti-TMP21 antibody [EPR9037(B)] (ab133771) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TMED10 knockout HEK293T cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab133771 observed at 19 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133771 Anti-TMP21 antibody [EPR9037(B)] was shown to specifically react with TMP21 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266227 (knockout cell lysate ab258233) was used. Wild-type and TMP21 knockout samples were subjected to SDS-PAGE. ab133771 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human TMED10 knockout HEK293T cell line (ab266227)Homozygous: 2 bp insertion in exon1 -
Human TMED10 (TMP21) knockout HEK293T cell line (ab266227)