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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Proto-oncogenes

Anti-SIX1 antibody [CL0185] (ab236761)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-SIX1 antibody [CL0185] (ab236761)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [CL0185] to SIX1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-SIX1 antibody [CL0185]
    See all SIX1 primary antibodies
  • Description

    Mouse monoclonal [CL0185] to SIX1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human SIX1 aa 100 to the C-terminus.
    Database link: Q15475

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: RH-30 cells. WB: RH-30 cell lysate. IHC-P: Human striated muscle tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CL0185
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation

Images

  • Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml

    Lane 1 : Control siRNA transfected RH-30 cell lysate
    Lane 2 : SIX1 siRNA probe 1 transfected RH-30 cell lysate
    Lane 3 : SIX1 siRNA probe 2 transfected RH-30 cell lysate

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)

    Formalin-fixed, paraffin-embedded human striated muscle tissue stained for SIX1 with ab236761 at a 1/200 dilution in immunohistochemical analysis.

    Shows strong nuclear immunoreactivity in the myocytes.

  • Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [CL0185] (ab236761)
    Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [CL0185] (ab236761)

    PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for SIX1 (Green) using ab236761 at 4 μg/ml in ICC/IF.

    Microtublues are stained red.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)

    Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for SIX1 when incubated with ab236761 at a 1/200 dilution (negative control).

  • Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml

    Lane 1 : RH-30 cell lysate
    Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

    Predicted band size: 32 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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