Anti-SIX1 antibody [CL0185] (ab236761)
Key features and details
- Mouse monoclonal [CL0185] to SIX1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SIX1 antibody [CL0185]
See all SIX1 primary antibodies -
Description
Mouse monoclonal [CL0185] to SIX1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human SIX1 aa 100 to the C-terminus.
Database link: Q15475 -
Positive control
- ICC/IF: RH-30 cells. WB: RH-30 cell lysate. IHC-P: Human striated muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0185 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml
Lane 1 : Control siRNA transfected RH-30 cell lysate
Lane 2 : SIX1 siRNA probe 1 transfected RH-30 cell lysate
Lane 3 : SIX1 siRNA probe 2 transfected RH-30 cell lysate
Predicted band size: 32 kDa
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Formalin-fixed, paraffin-embedded human striated muscle tissue stained for SIX1 with ab236761 at a 1/200 dilution in immunohistochemical analysis.
Shows strong nuclear immunoreactivity in the myocytes.
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PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for SIX1 (Green) using ab236761 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
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Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for SIX1 when incubated with ab236761 at a 1/200 dilution (negative control).
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All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml
Lane 1 : RH-30 cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 32 kDa