Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [1593] to SIX1
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-SIX1 antibody [1593]
See all SIX1 primary antibodies
Description

Mouse monoclonal [1593] to SIX1
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A-204 whole cell lysate. IHC-P: Human, mouse and rat skeletal muscle tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

1593
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-SIX1 antibody [1593] (ab243247)

All lanes : Anti-SIX1 antibody [1593] (ab243247) at 0.459 µg/ml

Lane 1 : A-204 (human muscle rhabdomyosarcoma), whole cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-SIX1 antibody [1593] (ab243247)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com