Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human salivary gland tissue labelling SIX1 with ab211359 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

All lanes : Anti-SIX1 antibody (ab211359) at 0.4 µg/ml

Lane 1 : Rh30 (human rhabdomyosarcoma cell line) transfected with control siRNA, cell lysate
Lane 2 : Rh30 transfected with target specific siRNA probe #1, cell lysate
Lane 3 : Rh30 transfected with target specific siRNA probe #2, cell lysate

Predicted band size: 32 kDa

Loading control: Anti-PPIB

Immunocytochemistry analysis of A431 (human epidermoid carcinoma) cells labelling SIX1 with ab211359 at 2 µg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling SIX1 with ab211359 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling SIX1 with ab211359 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SIX1 with ab211359 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.