Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1593] to SIX1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SIX1 antibody [1593] - BSA and Azide free
    See all SIX1 primary antibodies

  • Description

    Mouse monoclonal [1593] to SIX1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A-204 whole cell lysate. IHC-P: Human, mouse and rat skeletal muscle tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255754 is the carrier-free version of ab243247. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse skeletal muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This image was produced using the same antibody clone but in a different formulation ab243247, PBS, sodium azide, glycerol and BSA.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat skeletal muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This image was produced using the same antibody clone but in a different formulation ab243247, PBS, sodium azide, glycerol and BSA.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).

  • Western blot - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
    Western blot - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
    All lanes : Anti-SIX1 antibody [1593] (ab243247) at 0.459 µg/ml

    Lane 1 : A-204 (human muscle rhabdomyosarcoma), whole cell lysate
    Lane 2 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 32 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).

  • Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
    Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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