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Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1229] to SIX1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SIX1 antibody [1229] - BSA and Azide free
    See all SIX1 primary antibodies

  • Description

    Mouse monoclonal [1229] to SIX1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A-204 cell lysate. IHC-P: Human lung, prostatic hyperplasia and skeletal muscle tissue. ICC/IF: A-204 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255759 is the carrier-free version of ab252224. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)
    Western blot - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)
    All lanes : Anti-SIX1 antibody [1229] (ab252224) at 0.545 µg/ml

    Lane 1 : A-204 (human muscle rhabdomyosarcoma) whole cell lysate
    Lane 2 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 32 kDa


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)

    Formalin-fixed paraffin-embedded human lung tissue stained for SIX1 using ab252224 at 5.45µg/ml followed by a ready to use secondary antibody. 

    Nuclear staining on human lung tissue.

    The section was incubated with ab252224 for 30 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

  • Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)
    Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)

    4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-204 (human muscle rhabdomyosarcoma) cells stained for SIX1 (Green) using ab252224 at 10.9µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as a counterstain at 1/200 dilution. Confocal image showing nuclear staining in A-204 cell line.
    Negative control: Caco-2.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)

    Formalin-fixed paraffin-embedded human skeletal muscle tissue stained for SIX1 using ab252224 at 5.45µg/ml followed by a ready to use secondary antibody. 

    Nuclear staining on human skeletal muscle tissue.

    The section was incubated with ab252224 for 30 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)

    Formalin-fixed paraffin-embedded human prostatic hyperplasia tissue stained for SIX1 using ab252224 at 5.45µg/ml followed by a ready to use secondary antibody. 

    Nuclear staining on human prostatic hyperplasia.

    The section was incubated with ab252224 for 30 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

  • Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)
    Anti-SIX1 antibody [1229] - BSA and Azide free (ab255759)

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