Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FUBP3 antibody [EPR13174] (ab181025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13174] to FUBP3
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-FUBP3 antibody [EPR13174]
    See all FUBP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13174] to FUBP3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2 and MCF-7 cell lysate. Human liver and placenta tissue. HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025)
    Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025)
    All lanes : Anti-FUBP3 antibody [EPR13174] (ab181025) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Immunohistochemical analysis of paraffin embedded Human placenta tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (ab181025)
    Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Immunofluorescent analysis of acetone fixed HeLa cells labeling FUBP3 using ab181025 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)
    Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Lysate from HeLa cells was immunoprecipitated with ab181025 at a 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST

  • Anti-FUBP3 antibody [EPR13174] (ab181025)
    Anti-FUBP3 antibody [EPR13174] (ab181025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-FUBP3 antibody [EPR13174] (ab181025)

© 2021 Astana Biomed Group LLP. All rights reserved.