Anti-GTF2H1 antibody (ab272022)
Key features and details
- Rabbit polyclonal to GTF2H1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GTF2H1 antibody
See all GTF2H1 primary antibodies -
Description
Rabbit polyclonal to GTF2H1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GTF2H1 aa 250-300. The exact sequence is proprietary. NP_001135779.1
Database link: P32780 -
Positive control
- WB: HEK-293T, Jurkat and HeLa whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human testis tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (ab272022)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling GTF2H1 with ab272022 at a 1/1,000 dilution. Detection: DAB.
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Western blot - Anti-GTF2H1 antibody (ab272022)All lanes : Anti-GTF2H1 antibody (ab272022) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Predicted band size: 62 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-GTF2H1 antibody (ab272022)GTF2H1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab272022 at 6 μg/mg lysate. WB was performed using ab272022 at 1 µg/ml.
Lane 1: ab272022 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP.
Exposure time: 10 seconds.