Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16791] to PKC - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC antibody [EPR16791] - BSA and Azide free
    See all PKC primary antibodies

  • Description

    Rabbit monoclonal [EPR16791] to PKC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250109 is the carrier-free version of ab179523 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250109 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Recombinant PKC isoforms were tested for reactivity to ab179521 by western blot, and are marked as positive (+) or negative (-).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC beta 2 full length protein
    Lane 4 : Active human PKC gamma full length protein
    Lane 5 : Active human PKC delta full length protein
    Lane 6 : Active human PKC mu full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa


    Exposure time: 3 minutes


    This data was developed using ab179523, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

  • Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

    Lane 1 : Active human PKC eta full length protein
    Lane 2 : Active human PKC epsilon full length protein
    Lane 3 : Active human PKC theta full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa


    Exposure time: 5 seconds


    This data was developed using ab179523, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag.

  • Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/1000 dilution

    Lane 1 : Active human PKC zeta full length protein
    Lane 2 : Active human PKC iota full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa


    Exposure time: 3 minutes


    This data was developed using ab179523, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC zeta full length protein (ab60848) contains aa1-592 with GST-tag; Active human PKC iota full length protein (ab60850) contains aa1-596 with GST-tag.

  • Other - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Other - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    This data was developed using ab179523, the same antibody clone in a different buffer formulation.Recombinant PKC isoforms were tested for reactivity by western blot, and are marked as positive (+) or negative (-).
  • Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution + Human fetal heart tissue lysate at 1/10 dilution

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa


    Exposure time: 3 minutes


    This data was developed using ab179523, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/10000 dilution

    Lane 1 : Human fetal skin tissue lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : Mouse lung tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab179523, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKCs (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331. Newton AC, 1995. J. Biol. Chem. 270, 28495-28498).

  • Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Western blot - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab179523, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKCs (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331. Newton AC, 1995. J. Biol. Chem. 270, 28495-28498).

  • Immunoprecipitation - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Immunoprecipitation - Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    This data was developed using ab179523, the same antibody clone in a different buffer formulation.PKC was immunoprecipitated from 1 mg of Mouse lung whole cell lysate with ab179523 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179523 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Mouse lung whole cell lysate, 10µg (Input). Lane 2: ab179523 IP in Mouse lung whole cell lysate. Lane 3: Rabbit IgG,monoclonal[EPR25A]-Isotype Control (ab172730) instead of ab179523 in Mouse lung whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)
    Anti-PKC antibody [EPR16791] - BSA and Azide free (ab250109)

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