Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human PKC epsilon protein (ab151935)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Suitable for: HPLC, SDS-PAGE

Description

  • Product name

    Recombinant Human PKC epsilon protein
    See all PKC epsilon proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    ab151935 has purity greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. 0.2 µM filtered.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q02156

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      QELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVW LSKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHPFFKEIDWVLLEQK KIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFS YFGEDLMP

    • Predicted molecular weight

      18 kDa

    • Amino acids

      580 to 737

    • Specifications

    Our Abpromise guarantee covers the use of ab151935 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.40
      Constituents: 99% Phosphate Buffer, 0.02% DTT, 0.88% Sodium chloride

    General Info

    • Alternative names

      • KPCE_HUMAN
      • MGC125656
      • MGC125657
      • nPKC epsilon
      • nPKC-epsilon
      • PKCE
      • Pkcea
      • PRKCE
      • Protein kinase C epsilon
      • Protein kinase C epsilon type
      see all

    • Function

      This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.

    • Domain

      The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.

    • Post-translational
      modifications

      Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab151935? Please let us know so that we can cite the reference in this datasheet.

ab151935 has not yet been referenced specifically in any publications.

Preparation and Storage

  • KPCE_HUMAN
  • MGC125656
  • MGC125657

Images

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