Anti-FOXP3 antibody [236A/E7] (ab20034) at 1/1000 dilution + Human mammary gland lysate at 20 µg

Predicted band size: 47 kDa

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP3 with ab23004 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat AntiRabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

All lanes : Anti-FOXP3 antibody [236A/E7] (ab20034) at 1/1000 dilution

Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty
vector (vector control), containing a GFP-Myc-tag, whole cell lysate
Lane 2 : HEK-293 transfected with FOXP3 (WT) expression vector containing a GFP-Myc-tag, whole cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 47 kDa


Exposure time: 1 second

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling FOXP3 with ab23004 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat AntiRabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

Immunohistochemical analysis of human large and locally advanced breast cancers staining FOXP3 using ab20034. (a) Low level of FOXP3⁺, CTLA-4⁺ Treg infiltration (b) High level of FOXP3⁺ and CTLA-4⁺ Treg infiltration. (Itu: intratumoral Str: stromal)

This image was generated from the hybridoma version of the product.

All lanes : Anti-FOXP3 antibody [236A/E7] (ab20034) at 5 µg/ml

Lane 1 : HEK293T cell lysate
Lane 2 : HEk293T cell lysate overexpressing Human FOXP3
Lane 3 : Human tonsil tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 47 kDa

ab20034 detects Human FOXP3 protein at ~50 kDa in HEK293T cells overexpressing the protein. It also detects FOXP3 in Human tonsil tissue lysate, however this band is significantly weaker in endogenous conditions. Upon higher exposure, weak bands can also be observed in HEK293T cell lysate.

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-FOXP3 antibody [236A/E7] (ab20034; 5 microgram per mL) overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-mouse (green; 1:10000) for 1 hour at room temperature before imaging.

ab20034 staining FOXP3 in Cynomolgus Monkey Spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% donkey serum for 20 minutes at room temperature; antigen retrieval was by heat mediation using EDTA, pH9.0. Samples were incubated with primary antibody (1/100) for 30 minutes. A Biotin-conjugated Donkey anti-mouse polyclonal (1/2000) was used as the secondary antibody.

This image was generated from the hybridoma version of the product.

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FoxP3+ cells mainly accumulate centrally.

The formalin-fixed, paraffin-embedded blocks were cut into approximately

This image was generated from the hybridoma version of the product.

ab20034 staining FOXP3 in human colon tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocking with 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/50 and incubated with sample for 2 hours at 21°C. An Alexa Fluor^®488-conjugated rabbit polyclonal to mouse IgG was used undiluted as secondary antibody.

This image was generated from the hybridoma version of the product.

See Abreview