Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23505-13] to Tau (phospho T205) - BSA and Azide free
  • Suitable for: WB, IHC-Fr, Dot blot, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR23505-13] to Tau (phospho T205) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Rat
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate; Mouse brain tissue lysate; rat hippocampus tissue lysate. IHC-P: Human Alzheimer's disease cerebrum and breast tissue; Rat cerebrum tissue. IHC-Fr: Rat cerebrum tissue; Mouse cerebrum tissue. IP: Rat brain tissue lysate; Mouse brain tissue lysate.

  • General notes

    ab275027 is the carrier-free version of ab254410. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275027 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Tau (phospho T205) with ab254410 at 1/20000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum without alkaline phosphatase treatment (image A, PMID: 28035925). No signal was detected when tissues were treated with alkaline phosphatase (image B).The section was incubated with ab254410 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Tau (phospho T205) with ab254410 at 1/500 (1.034 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum, while nearly no staining on mouse cerebrum after alkaline phosphatase (AP) treatment. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Western blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    All lanes : Anti-Tau (phospho T205) antibody [EPR23505-13] (ab254410) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate (phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 50-70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21722209).

  • Dot Blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Dot Blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Dot blot analysis of Tau (phospho T205) labeled with ab254410 at 1/1000 dilution.

    Lane 1: Tau (phospho S202+T205) peptide (aa 199-211).

    Lane 2: Tau (phospho S202+T205) peptide (aa 197-209).

    Lane 3: Tau peptide (aa 197-211).

    Lane 4: Tau (phospho S202) peptide (aa 197-211).

    Lane 5: Tau (phospho T205) peptide (aa 197-211).

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Immunoprecipitation - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Tau (phospho T205) was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab254410 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254410 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 ug

    Lane 2: ab254410 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254410 in Rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Tau (phospho T205) with ab254410 at 1/500 (1.034 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum, while nearly no staining on rat cerebrum after alkaline phosphatase (AP) treatment. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Western blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    All lanes : Anti-Tau (phospho T205) antibody [EPR23505-13] (ab254410) at 1/1000 dilution

    Lane 1 : Rat hippocampus tissue lysate
    Lane 2 : Rat hippocampus tissue lysate (phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?



    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 48 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21722209).

  • Western blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Western blot - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    All lanes : Anti-Tau (phospho T205) antibody [EPR23505-13] (ab254410) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate (phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?



    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 70 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21722209).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Tau (phospho T205) with ab254410 at 1/20000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast without alkaline phosphatase treatment (image A, PMID: 15914550). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab254410 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

  • Immunoprecipitation - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Immunoprecipitation - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Tau (phospho T205) was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab254410 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254410 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab254410 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254410 in Mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

    This data was developed using ab254410, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease cerebrum tissue labeling Tau (phospho T205) with ab254410 at 1/20000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human Alzheimer's disease cerebrum without alkaline phosphatase treatment (image A, PMID: 20631843). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab254410 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

  • Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)
    Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free (ab275027)

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