Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
![Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)](https://www.abcam.com/ps/products/206/ab206292/Images/ab206292-250221-anti-14-3-3-eta-antibody-epr16750-western-blot.jpg "Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-14-3-3 eta/YWHAH antibody [EPR16750]
See all 14-3-3 eta/YWHAH primary antibodies -
Description
Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseRatHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain lysate; Jurkat, HeLa, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain, spleen, heart and kidney lysates; HeLa cytosolic and nuclear fractions. IP: Human brain whole cell lysate.
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General notes
This product was previously labelled as 14-3-3 eta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16750 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2 and 3: 3 minutes.
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![Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)](https://www.abcam.com/ps/products/206/ab206292/Images/ab206292-250220-anti-14-3-3-eta-antibody-epr16750-western-blot.jpg)
Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Mouse heart lysate
Lane 8 : Mouse kidney lysate
Lane 9 : Rat heart lysate
Lane 10 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1- 6 : 30 seconds; Lane 7 -10 : 3 minutes.
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![Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)](https://www.abcam.com/ps/products/206/ab206292/Images/ab206292-250219-anti-14-3-3-eta-antibody-epr16750-western-blot.jpg)
Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)](https://www.abcam.com/ps/products/206/ab206292/Images/ab206292-250217-anti-14-3-3-eta-antibody-epr16750-immunoprecipitation.jpg)
Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)](https://www.abcam.com/ps/products/206/ab206292/Images/ab206292-250218-anti-14-3-3-eta-antibody-epr16750-immunoprecipitation.jpg)
Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)Negative control: 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)](https://www.abcam.com/ps/products/206/ab206292/Images/ab206292-7-benefits-of-recombinant-antibodies.png)
Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
