Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22709-52] to Iroquois homeobox protein 3/IRX3
Suitable for: WB, Flow Cyt, ICC, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52]
See all Iroquois homeobox protein 3/IRX3 primary antibodies
Description

Rabbit monoclonal [EPR22709-52] to Iroquois homeobox protein 3/IRX3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IHC-P	Mouse Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse embryo, E14.5 lung and E17 lung tissue lysates; Rat E17 brain (primary culture) tissue lysate. IHC-P: Mouse E14.5 heart and E14.5 lung tissue; Rat E14.5 heart tissue. ICC: HEK-293T cells. Flow cyt: HEK-293T cells (transfected with Flag-tagged IRX3 expression vector).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22709-52
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells ( transfected with Flag-tagged IRX3 expression vector) labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with Flag-tagged IRX3 expression vector. is observed. Anti-FLAG mouse monoclonal antibody was used at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 lung tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lung of mouse E14.5 (PMID: 11520674 ). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Flow Cytometry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with Flag-tagged IRX3 expression vector) cells labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

Lane 1 : Mouse E17 embryo tissue lysate
Lane 2 : Rat E17 brain (primary culture) tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 52 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 125 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

Immunohistochemical analysis of paraffin-embedded Rat E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of rat E14.5. The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

Lane 1 : Mouse embryo tissue lysate
Lane 2 : Mouse E14.5 lung tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of mouse E14.5 (PMID: 15342473, PMID: 11520674). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

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