Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22709-52] to Iroquois homeobox protein 3/IRX3
  • Suitable for: WB, Flow Cyt, ICC, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52]
    See all Iroquois homeobox protein 3/IRX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22709-52] to Iroquois homeobox protein 3/IRX3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse embryo, E14.5 lung and E17 lung tissue lysates; Rat E17 brain (primary culture) tissue lysate. IHC-P: Mouse E14.5 heart and E14.5 lung tissue; Rat E14.5 heart tissue. ICC: HEK-293T cells. Flow cyt: HEK-293T cells (transfected with Flag-tagged IRX3 expression vector).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22709-52

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Immunocytochemistry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells ( transfected with Flag-tagged IRX3 expression vector) labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with Flag-tagged IRX3 expression vector. is observed. Anti-FLAG mouse monoclonal antibody was used at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

    Immunohistochemical analysis of paraffin-embedded Mouse E14.5 lung tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lung of mouse E14.5 (PMID: 11520674 ). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Flow Cytometry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with Flag-tagged IRX3 expression vector) cells labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

    Lane 1 : Mouse E17 embryo tissue lysate
    Lane 2 : Rat E17 brain (primary culture) tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 125 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

    Immunohistochemical analysis of paraffin-embedded Rat E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of rat E14.5. The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

    Lane 1 : Mouse embryo tissue lysate
    Lane 2 : Mouse E14.5 lung tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

    Immunohistochemical analysis of paraffin-embedded Mouse E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of mouse E14.5 (PMID: 15342473, PMID: 11520674). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)
    Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133)

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