Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free (ab249266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10862(B)] to ACADS/SCAD - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free
    See all ACADS/SCAD primary antibodies

  • Description

    Rabbit monoclonal [EPR10862(B)] to ACADS/SCAD - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human hepatocellular carcinoma, Human heart and Human thyroid gland tissues tissue. IP: Human liver cells.

  • General notes

    Ab249266 is the carrier-free version of ab156571. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249266 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Immunoprecipitation - Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free (ab249266)
    Immunoprecipitation - Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free (ab249266)

    ab156571 (Purified) at 1:20 dilution (2 µg) immunoprecipitating ACADS/SCAD in Human liver lysate. 
    Lane 1 (input): Human liver lysate 10 µg
    Lane 2 (+): ab156571 & Human liver lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab156571 in Human liver lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156571)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free (ab249266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free (ab249266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling ACADS/SCAD with Purified ab156571 at 1:100 dilution (3.76 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156571)
  • Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free (ab249266)
    Anti-ACADS/SCAD antibody [EPR10862(B)] - BSA and Azide free (ab249266)

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