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Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23695-115] to Cdk7 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free
    See all Cdk7 primary antibodies

  • Description

    Rabbit monoclonal [EPR23695-115] to Cdk7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details
    Unsuitable for: ChIP or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, NIH/3T3, RAW 264.7, C6, HepG2, HCT 116 and MEF whole cell lysates; Mouse spleen tissue lysate; Rat spleen tissue lysate. IHC-P: Mouse testis and breast cancer tissue; Rat testis tissue. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: NIH/3T3 whole cell lysate.

  • General notes

    ab273630 is the carrier-free version of ab256787.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Cdk7 with ab256787 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat testis (PMID: 11319144, PMID: 8521393). The section was incubated with ab256787 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256787).

  • Immunoprecipitation - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)
    Immunoprecipitation - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

    Cdk7 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab256787 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256787 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: ab256787 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256787 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256787).

  • Flow Cytometry - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)
    Flow Cytometry - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Cdk7 with ab256787 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256787).

  • Flow Cytometry - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)
    Flow Cytometry - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Cdk7 with ab256787 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256787).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

    Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling Cdk7 with ab256787 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse breast cancer (PMID: 27301701). The section was incubated with ab256787 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256787).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Cdk7 with ab256787 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse testis (PMID: 11319144, PMID: 8521393). The section was incubated with ab256787 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256787).

  • Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)
    Anti-Cdk7 antibody [EPR23695-115] - BSA and Azide free (ab273630)

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