Anti-p27 KIP 1 antibody (ab45872)
Key features and details
- Goat polyclonal to p27 KIP 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p27 KIP 1 antibody
See all p27 KIP 1 primary antibodies -
Description
Goat polyclonal to p27 KIP 1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cat, Dog, Pig, Duck
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Immunogen
Synthetic peptide corresponding to Human p27 KIP 1 aa 153-165 (internal sequence).
Sequence:C-KRPATDDSSTQNK
Database link: P46527 -
Positive control
- WB: Human prostate tissue lysate. IHC-P: Human prostate and spleen tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p27 KIP 1 antibody (ab45872)Anti-p27 KIP 1 antibody (ab45872) at 0.1 µg/ml + Human Prostate lysate (35µg protein in RIPA buffer).
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (ab45872)ab45872 at 5µg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (ab45872)Immunohistochemical analysis of paraffin embedded human spleen labeling p27 KIP 1 with ab45872 at 5µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
