Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] - BSA and Azide free (ab247389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3224] to PACT (PKR activating protein) / PRKRA - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] - BSA and Azide free
    See all PACT (PKR activating protein) / PRKRA primary antibodies

  • Description

    Rabbit monoclonal [EPR3224] to PACT (PKR activating protein) / PRKRA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, K562 and HepG2 cell lysates.

  • General notes

    Ab247389 is the carrier-free version of ab75749. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247389 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] - BSA and Azide free (ab247389)
    Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] - BSA and Azide free (ab247389)
    All lanes : Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] (ab75749) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PRKRA knockout HeLa cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab75749).

    Lanes 1-4: Merged signal (red and green). Green - ab75749 observed at 36 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab75749 Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] was shown to specifically react with PACT in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266806 (knockout cell lysate ab258141) was used.  Wild-type and PACT knockout samples were subjected to SDS-PAGE.  ab75749 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] - BSA and Azide free (ab247389)
    Anti-PACT (PKR activating protein) / PRKRA antibody [EPR3224] - BSA and Azide free (ab247389)

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