Anti-PFD6 antibody (ab184984)
Key features and details
- Rabbit polyclonal to PFD6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PFD6 antibody -
Description
Rabbit polyclonal to PFD6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Recombinant fragment corresponding to Human PFD6 aa 50 to the C-terminus.
Database link: O15212 -
Positive control
- IHC-P: Human colon, liver, pancreas and testis tissue; WB: RT-4 and U-251 MG sp whole cell lysate; ICC: U-2 OS whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)
Immunohistochemistry analysis of human testis tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-PFD6 antibody (ab184984)All lanes : Anti-PFD6 antibody (ab184984) at 1/500 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) whole cell lysate
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (ab184984)Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling PFG6 at the nucleus and the Golgi apparatus with ab184984 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)Immunohistochemistry analysis of human pancreas tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)Immunohistochemistry analysis of human colon tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)Immunohistochemistry analysis of human liver tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.