Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Dicer antibody [EPR24104-105] (ab259327)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24104-105] to Dicer
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Dicer antibody [EPR24104-105]
See all Dicer primary antibodies
Description

Rabbit monoclonal [EPR24104-105] to Dicer
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: RAW264.7, 2.4G2, HeLa, 293T and NIH/3T3, PC-12 lysates. IHC-P: Human testis, Mouse cerebrum and testis, Rat testis tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24104-105
Isotype

IgG
Research areas

Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)

All lanes : Anti-Dicer antibody [EPR24104-105] (ab259327) at 1/1000 dilution

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 218 kDa
Observed band size: 250 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: THP-1 (PMID:20584909).

Lysates were made freshly and used in WB test immediately to minimize protein degradation.

Exposure time: 48 seconds
Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)

All lanes : Anti-Dicer antibody [EPR24104-105] (ab259327) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 218 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Lysates were made freshly and used in WB test immediately to minimize protein degradation.

Exposure time: Lane 1, 3, 4: 3 minutes

Lane 2: 26 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID:17573847). The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID:32737764). The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis. The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-Dicer antibody [EPR24104-105] (ab259327)

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