Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 123 964 ₸

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Anti-GFPT2 antibody [EPR19095] (ab190966)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19095] to GFPT2
Suitable for: WB, ICC/IF, IP, Flow Cyt
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-GFPT2 antibody [EPR19095]
See all GFPT2 primary antibodies
Description

Rabbit monoclonal [EPR19095] to GFPT2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Mouse
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human GFPT2 recombinant protein fragment; Human placenta and glioma lysates; Mouse placenta, heart, kidney and spleen lysates; Rat placenta, heart and spleen lysates; A-375, U-87MG and NIH/3T3 whole cell lysates. ICC/IF: U-87 MG cell line. Flow Cyt: U-87 MG cell line. IP: NIH/3T3 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19095
Isotype

IgG
Research areas

Western blot - Anti-GFPT2 antibody [EPR19095] (ab190966)

All lanes : Anti-GFPT2 antibody [EPR19095] (ab190966) at 1/2000 dilution

Lane 1 : Human GFPT2 recombinant protein fragment
Lane 2 : Human GFPT1 recombinant protein fragment

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 77 kDa
Observed band size: 33 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.
Human GFPT2 recombinant protein fragment contains aa2-305 with a His-Tag®, Human GFPT1 recombinant protein fragment contains aa2-288 with a His-Tag®.
Western blot - Anti-GFPT2 antibody [EPR19095] (ab190966)

All lanes : Anti-GFPT2 antibody [EPR19095] (ab190966) at 1/2000 dilution

Lane 1 : Human placenta lysate
Lane 2 : Mouse placenta lysate
Lane 3 : A-375 (Human malignant melanoma cell line) whole cell lysate
Lane 4 : Rat placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 & 2: 3 minutes.
Lane 3 & 4: 5 seconds.
Western blot - Anti-GFPT2 antibody [EPR19095] (ab190966)

All lanes : Anti-GFPT2 antibody [EPR19095] (ab190966) at 1/2000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat spleen lysate
Lane 6 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-GFPT2 antibody [EPR19095] (ab190966)

All lanes : Anti-GFPT2 antibody [EPR19095] (ab190966) at 1/2000 dilution

Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : Human glioma lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-GFPT2 antibody [EPR19095] (ab190966)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling GFPT2 with ab190966 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasm staining on U-87 MG cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab190966 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Flow Cytometry - Anti-GFPT2 antibody [EPR19095] (ab190966)

Flow cytometric analysis of 4% paraformaldehyde-fixed U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling GFPT2 with ab190966 at 1/150 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue).
Goat anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-GFPT2 antibody [EPR19095] (ab190966)

GFPT2 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate with ab190966 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab190966 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10ug (Input).

Lane 2: ab190966 IP in NIH/3T3 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190966 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5 seconds.
Anti-GFPT2 antibody [EPR19095] (ab190966)

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