Anti-SHMT1 antibody (ab186130)
Key features and details
- Rabbit polyclonal to SHMT1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SHMT1 antibody
See all SHMT1 primary antibodies -
Description
Rabbit polyclonal to SHMT1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human SHMT1 aa 450 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_004160.3).
Database link: P34896 -
Positive control
- Jurkat and NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab186130 was affinity purified using an epitope specific to SHMT1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHMT1 antibody (ab186130)Anti-SHMT1 antibody (ab186130) at 1 µg/ml + Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes
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Western blot - Anti-SHMT1 antibody (ab186130)Anti-SHMT1 antibody (ab186130) at 1 µg/ml + NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-SHMT1 antibody (ab186130)Detection of SHMT1 in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186130 at 6 µg per reaction for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
