Anti-MSRA antibody (ab16803)
Key features and details
- Rabbit polyclonal to MSRA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MSRA antibody
See all MSRA primary antibodies -
Description
Rabbit polyclonal to MSRA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Macaque monkey
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Immunogen
Recombinant full length protein corresponding to Human MSRA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MSRA antibody (ab16803)All lanes : Anti-MSRA antibody (ab16803)
Lane 1 : Mouse Kidney
Lane 2 : Rat Kidney
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Western blot using ab16803 at 1/2000.
Lane 1: Mouse kidney
Lane 2: Rat kidney -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSRA antibody (ab16803)ab16803 (1µg/ml) staining MSRA in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Western blot - Anti-MSRA antibody (ab16803)
