Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CTPS2 antibody [EPR16735] (ab196016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16735] to CTPS2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CTPS2 antibody [EPR16735]
    See all CTPS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16735] to CTPS2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, 293, Raw264.7 and PC12 cell lysates; IHC-P: Human transitional cell carcinoma of bladder, Human stomach tissues; ICC/IF: K562 cells; Flow cytometry: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/20000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)
    All lanes : Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/2000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 2 : 293 (Human epithelial cells from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)
    All lanes : Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/2000 dilution

    Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTPS2 antibody [EPR16735] (ab196016)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling CTPS2 using ab196016 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and Nuclear staining is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    The staining pattern observed is consistent with what has been described in the literature PMID: 24870241 and PMID: 16179339.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTPS2 antibody [EPR16735] (ab196016)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CTPS2 using ab196016 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and Nuclear staining is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    The staining pattern observed is consistent with what has been described in the literature PMID: 24870241 and PMID: 16179339.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Immunocytochemistry/ Immunofluorescence - Anti-CTPS2 antibody [EPR16735] (ab196016)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling CTPS2 with ab196016 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic and Nuclear staining is observed. The nuclear counter stain is DAPI (blue).
    Negative control obtained using PBS instead of ab196016 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    The staining pattern observed is consistent with what has been described in the literature PMID: 16179339 and 24870241.

  • Flow Cytometry - Anti-CTPS2 antibody [EPR16735] (ab196016)
    Flow Cytometry - Anti-CTPS2 antibody [EPR16735] (ab196016)

    Flow cytometry analysis of HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CTPS2 using ab196016 at 1/540 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).

  • Anti-CTPS2 antibody [EPR16735] (ab196016)
    Anti-CTPS2 antibody [EPR16735] (ab196016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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