Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CTPS2 antibody [EPR16735] (ab196016)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16735] to CTPS2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-CTPS2 antibody [EPR16735]
See all CTPS2 primary antibodies
Description

Rabbit monoclonal [EPR16735] to CTPS2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HepG2, 293, Raw264.7 and PC12 cell lysates; IHC-P: Human transitional cell carcinoma of bladder, Human stomach tissues; ICC/IF: K562 cells; Flow cytometry: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16735
Isotype

IgG
Research areas

Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)

Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/20000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)

All lanes : Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/2000 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
Lane 2 : 293 (Human epithelial cells from embryonic kidney) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-CTPS2 antibody [EPR16735] (ab196016)

All lanes : Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/2000 dilution

Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

5% NFDM/TBST: Blocking and diluting buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTPS2 antibody [EPR16735] (ab196016)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling CTPS2 using ab196016 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and Nuclear staining is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

The staining pattern observed is consistent with what has been described in the literature PMID: 24870241 and PMID: 16179339.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTPS2 antibody [EPR16735] (ab196016)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CTPS2 using ab196016 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and Nuclear staining is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

The staining pattern observed is consistent with what has been described in the literature PMID: 24870241 and PMID: 16179339.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CTPS2 antibody [EPR16735] (ab196016)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling CTPS2 with ab196016 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic and Nuclear staining is observed. The nuclear counter stain is DAPI (blue).
Negative control obtained using PBS instead of ab196016 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.
The staining pattern observed is consistent with what has been described in the literature PMID: 16179339 and 24870241.
Flow Cytometry - Anti-CTPS2 antibody [EPR16735] (ab196016)

Flow cytometry analysis of HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CTPS2 using ab196016 at 1/540 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).
Anti-CTPS2 antibody [EPR16735] (ab196016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com