Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CLIC2 antibody [EPR6494] (ab175230)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6494] to CLIC2
Suitable for: ICC/IF, IP, IHC-P, WB
Reacts with: Human

Product name

Anti-CLIC2 antibody [EPR6494]
See all CLIC2 primary antibodies
Description

Rabbit monoclonal [EPR6494] to CLIC2
Host species

Rabbit
Specificity

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CLIC2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O15247
Positive control
- Human fetal liver, Human heart and Human testis lysates; Human liver tissue; HepG2 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6494
Isotype

IgG
Research areas

Western blot - Anti-CLIC2 antibody [EPR6494] (ab175230)

All lanes : Anti-CLIC2 antibody [EPR6494] (ab175230) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human heart lysate
Lane 3 : Human testis lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 28 kDa
Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (ab175230)

Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] (ab175230)

Immunofluorescent analysis of HepG2 cells labeling CLIC2 with ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] (ab175230)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with ab175230 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CLIC2 antibody [EPR6494] (ab175230)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com