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Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL7] to SLAMF7/CS1 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free
    See all SLAMF7/CS1 primary antibodies

  • Description

    Rabbit monoclonal [CAL7] to SLAMF7/CS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human SLAMF7/CS1 aa 200 to the C-terminus.
    Database link: Q9NQ25

  • Positive control

    • IHC-P: SLAMF7/CS1 Knock-in AD-293 cells; OPM2 xenograft tissue. IP: Human tonsil and IM-9 lysate

  • General notes

    ab251615 is the carrier-free version of ab237730 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251615 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Clone also known as 43H1L46 This product was previously labelled as SLAMF7

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purity is greater than 99%.

  • Clonality

    Monoclonal

  • Clone number

    CAL7

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human tonsil is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237730).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)

    Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human multiple myeloma is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237730).

  • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)
    Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)

    SLAMF7/CS1 was immunoprecipitated from 0.35mg of IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.

    Lane 1: Human tonsil lysate 10μg (Input)

    Lane 2: ab237730 IP in Human tonsil lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237730 in IM-9 whole cell lysate

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237730).

  • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)
    Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)

    SLAMF7/CS1 was immunoprecipitated from 0.35mg of IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.

    Lane 1: IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate 10μg (Input)

    Lane 2: ab237730 IP in IM-9 lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237730 in IM-9 whole cell lysate

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237730).

  • Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)
    Anti-SLAMF7/CS1 antibody [CAL7] - BSA and Azide free (ab251615)

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