Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies

  • Description

    Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for Glutamate Receptor 1 (AMPA subtype).

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: SH-SY5Y whole cell lysate.

  • General notes

    ab247259 is the carrier-free version of ab32436 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247259 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution + SH-SY5Y cell lysate

    Predicted band size: 101 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32436, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)
    This data was developed using ab32436, the same antibody clone in a different buffer formulation.Ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry.
  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)
    Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)

    This data was developed using ab32436, the same antibody clone in a different buffer formulation.
    Purified ab32436 at 1/50 dilution (2µg) immunoprecipitating Glutamate Receptor 1 (AMPA subtype) in SH-SY5Y whole cell lysate.
    Lane 1 (input): SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab32436 + SH-SY5Y whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32436 in SH-SY5Y whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 106 kDa

  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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