Glutamate Oxidase Assay Kit (Fluorometric - Near Infrared) (ab138885)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Cell Lysate
- Sensitivity: 40 uU/ml
Overview
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Product name
Glutamate Oxidase Assay Kit (Fluorometric - Near Infrared) -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell Lysate -
Assay type
Enzyme activity -
Sensitivity
= 40 uU/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Glutamate Oxidase Assay Kit (Fluorometric - Near Infrared) (ab138885) provides a quick and an ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to a-ketoglutarate, NH3 and H2O2 by glutamate oxidase. The kit uses our AbRed Indicator substrate which can react with H2O2 when catalyzed by horseradish peroxidase (HRP) to generate the highly fluorescent product. The signal can be read with either a fluorescence microplate reader at Ex/Em = 530-570 nm/590 -600 nm (optimal Ex/Em = 540 nm/590 nm) or an absorbance microplate reader at 576±5 nm. With this product, we have detected as little as 40 µU/mL glutamate oxidase in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
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Notes
Glutamate oxidase specifically catalyzes the oxidative deamination of L-glutamate in the presence of water and oxygen with the formation of ketoglutarate, ammonia, and hydrogen peroxide. Glutamate oxidase can be used as an analytic reagent and as a basis for developing biosensors for the determination of L-glutamate, L-glutamine, ammonia, and creatinine. These biosensors can be used in clinical biochemistry for the determination of glutamate-pyruvate transaminase and glutamate-oxalacetate transaminase in biological fluids, which makes the early diagnosis of heart and liver diseases possible.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests AbRed Indicator 1 vial Assay Buffer 1 x 20ml DMSO 1 x 200µl Glutamate Oxidase Standard 1 vial Glutamic Acid 1 x 3.4mg Horseradish Peroxidase 1 vial -
Research areas
Images
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Sample Standard Curve for Glutamate OxidaseGlutamate oxidase dose response was measured on a 96- well black plate with ab138885. As low as 40 µU/mL glutamate oxidase can be detected with 60 minutes incubation time (n=3).