Anti-ISG20 antibody (ab154393)
Key features and details
- Rabbit polyclonal to ISG20
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ISG20 antibody
See all ISG20 primary antibodies -
Description
Rabbit polyclonal to ISG20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-181 of Human ISG20 (Q96AZ6).
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Positive control
- Raji whole cell lysate. HeLa cells. BT483 xenograft.
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-ISG20 antibody (ab154393) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 20 kDa
12% SDS PAGE -
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human non-Hodgkin lymphoma tissue sections, labelling ISG20 with ab154393 at a dilution of 1/50 incubated for 12 hours at 4°C. Heat mediated antigen retrival was with citrate buffer at pH 6.0 incubated for 15 minutes. Blocking was a with 5% serum incubated for 30 minutes at 25°C. Secondary was a Goat anti-rabbit polyclonal CY3® conjugate at 1/200. Counterstain was with DAPI against nuclear DNA.
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Immunohistochemical analysis of paraffin-embedded BT483 xenograft, labelling ISG20 with ab154393 at 1/500 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling ISG20 with ab154393 at a 1/500 dilution. Co-stained with Hoechst 33342.