Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)
![Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)](https://www.abcam.com/ps/products/272/ab272225/Images/ab272225-387956-anti-irf7-antibody-epr23509-21-immunoprecipitation-human.jpg "Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23509-21] to IRF7 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free
See all IRF7 primary antibodies -
Description
Rabbit monoclonal [EPR23509-21] to IRF7 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate. IP: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate.
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General notes
ab272225 is the carrier-free version of ab238137. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23509-21 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)
IRF7 was immunoprecipitated from 0.35 mg of Jurkat (treated with 100 nM IFN alpha for 18 hours, then added 10 μM MG-132 for another 6 hours) whole cell lysate with ab238137 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab238137 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Jurkat (treated as above) whole cell lysate 10 μg (Input).
Lane 2: ab238137 IP in Jurkat (treated as above) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238137 in Jurkat (treated as above) whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 seconds.65-kDa full length IRF7 and 45-kDa C-terminal cleaved IRF7 are observed (PMID: 23175366).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab238137).
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![Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)](https://www.abcam.com/ps/products/272/ab272225/Images/ab272225-2-benefits-of-recombinant-antibodies.png)
Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)
