Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23509-21] to IRF7 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free
    See all IRF7 primary antibodies

  • Description

    Rabbit monoclonal [EPR23509-21] to IRF7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate. IP: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate.

  • General notes

    ab272225 is the carrier-free version of ab238137. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)
    Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)

    IRF7 was immunoprecipitated from 0.35 mg of Jurkat (treated with 100 nM IFN alpha for 18 hours, then added 10 μM MG-132 for another 6 hours) whole cell lysate with ab238137 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab238137 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Jurkat (treated as above) whole cell lysate 10 μg (Input). 
    Lane 2: ab238137 IP in Jurkat (treated as above) whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238137 in Jurkat (treated as above) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 84 seconds.

    65-kDa full length IRF7 and 45-kDa C-terminal cleaved IRF7 are observed (PMID: 23175366).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab238137).

  • Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)
    Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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