Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free
See all LPCAT1 primary antibodies
Description

Rabbit monoclonal [EPR19882] to LPCAT1 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251545 is the carrier-free version of ab214034. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251545 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR19882
Isotype

IgG

Western blot - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

Lanes 1-6 : Anti-LPCAT1 antibody [EPR19882] (ab214034) at 1/2000 dilution
Lanes 7-8 : Anti-LPCAT1 antibody [EPR19882] (ab214034) at 1/10000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human testis lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 5 : Human fetal spleen lysate
Lane 6 : Human fetal kidney lysate
Lane 7 : Human prostate cancer lysate
Lane 8 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

This data was developed using ab214034, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: Lanes 1-4, and 8: 4 seconds; Lanes 5-6: 8 seconds; Lane 7: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic or granular staining on the type two alveolar epithelial cells of human lung is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic or granular staining on the liver cancer cells which contain abundant lipid drops is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic or granular staining on the lung cancer cells is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A431 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A549 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Flow Cytometry - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (Human lung carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/50 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed A431 (Human epidermoid carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)

This data was developed using ab214034, the same antibody clone in a different buffer formulation.LPCAT1 was immunoprecipitated from 0.35 mg of A549 (Human lung carcinoma cell line) whole cell lysate with ab214034 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214034 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution Lane 1: A549 whole cell lysate 10µg (Input). Lane 2: ab214034 IP in A549 whole cell lysate. Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214034 in A549 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
Anti-LPCAT1 antibody [EPR19882] - BSA and Azide free (ab251545)