Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon gamma antibody [EPR1108] (ab133566)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1108] to Interferon gamma
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Interferon gamma antibody [EPR1108]
    See all Interferon gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR1108] to Interferon gamma

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Interferon gamma aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P01579

  • Positive control

    • WB: Recombinant Interferon gamma protein; MOLT4 and Jurkat cell lysates; Human ymph node and kidney lysates. Flow cyt: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    All lanes : Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified)

    Lane 1 : MOLT4 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Lymph node lysate
    Lane 4 : Human kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa

  • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 19 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 19 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Molt-4 at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 19 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified) + Recombinant Interferon gamma protein at 0.005 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa



    Note: Molecular weight of recombinant protein is 17 kDa.
  • Anti-Interferon gamma antibody [EPR1108] (ab133566)
    Anti-Interferon gamma antibody [EPR1108] (ab133566)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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