Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Interferon gamma antibody [EPR1108] (ab133566)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1108] to Interferon gamma
Suitable for: WB
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-Interferon gamma antibody [EPR1108]
See all Interferon gamma primary antibodies
Description

Rabbit monoclonal [EPR1108] to Interferon gamma
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide within Human Interferon gamma aa 1-100 (internal sequence). The exact sequence is proprietary.
Database link: P01579
Positive control
- WB: Recombinant Interferon gamma protein; MOLT4 and Jurkat cell lysates; Human ymph node and kidney lysates. Flow cyt: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1108
Isotype

IgG
Research areas

Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

All lanes : Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified)

Lane 1 : MOLT4 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Lymph node lysate
Lane 4 : Human kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Rat brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 19 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 19 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Molt-4 at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 19 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified) + Recombinant Interferon gamma protein at 0.005 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

Note: Molecular weight of recombinant protein is 17 kDa.
Anti-Interferon gamma antibody [EPR1108] (ab133566)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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